ABSTRACT
It was previously demonstrated that the methanol fraction of Sideroxylon obtusifolium (MFSOL) promoted anti-inflammatory and healing activity in excisional wounds. Thus, the present work investigated the healing effects of MFSOL on human keratinocyte cells (HaCaT) and experimental burn model injuries. HaCaT cells were used to study MFSOL's effect on cell migration and proliferation rates. Female Swiss mice were subjected to a second-degree superficial burn protocol and divided into four treatment groups: Vehicle, 1.0% silver sulfadiazine, and 0.5 or 1.0% MFSOL Cream (CrMFSOL). Samples were collected to quantify the inflammatory mediators, and histological analyses were performed after 3, 7, and 14 days. The results showed that MFSOL (50 μg/mL) stimulated HaCaT cells by increasing proliferation and migration rates. Moreover, 0.5% CrMFSOL attenuated myeloperoxidase (MPO) activity and also stimulated the release of interleukin (IL)-1β and IL-10 after 3 days of treatment. CrMFSOL (0.5%) also enhanced wound contraction, promoted improvement of tissue remodeling, and increased collagen production after 7 days and VEGF release after 14 days. Therefore, MFSOL stimulated human keratinocyte (HaCaT) cells and improved wound healing via modulation of inflammatory mediators of burn injuries.
Subject(s)
Humans , Female , Burns/drug therapy , Sapotaceae , Proline , Keratinocytes , Plant Leaves , MethanolABSTRACT
OBJECTIVE：To compare the chemical compo sition difference in methanol and petroleum ether fraction from Curcuma longa of different habitats. METHODS ：The ultrasonic method was used to extract C. longa from 7 defferent producingareas（S1-S7），and methanol and petroleum ether fraction were obtained and calculated yield. The curcumin compounds in methanol fraction were determined by LC-MS ；The chemical components in petroleum ether fraction were analyzed by GC-MS ， and the relative percentage content was determined by peak area normalization method after determining its structure by comparing NIST 2005 standard mass spectra and Wiley 275 standard mass spectra. SPSS 25.0 software was used for principle component analysis（PCA）and cluster analysis of relative percentage content of common components in petroleum ether fraction from C. longa of different habitats. At the same time ，the influence of latitude of the habitats on the content of total tumerone （by tumerone and ar-tumerone ）was analyzed. RESULTS ：The yield of methanol fraction were 1.35％-8.90％ from C. longa of 7 habitats；the yield of petroleum ether fraction were 0.81％-4.90％，which were the highest in C. longa from Longyan of Fujian Province. There was no significant difference in the relative content of curcumin compounds（reference peak area ）from S 1，S3-S7，which was in descending order as follows as curcumin ＞desmethoxycurcumin＞bisdemethoxycurcumin. There was slightly different in curcumin compounds of C. longa from S 2，mainly manifesting as the content of bisdemethoxycurcumin was higher than that from other producing areas. Totally 48 chemical compositions were identified from petroleum ether fraction in C. longa from different habitats ， mainly being sesquiterpenoids and monoterpenoids. 23，10，15，18，11，14，15 chemical compositions were identified from S1-S7，accounting for 94.49％，96.09％，95.66％，98.98％，99.24％，89.05％ and 97.27％. There were 4 common compositions in C. longa from different habitats ，which were tumerone （17.90％-43.07％），ar-tumerone（6.97％-33.66％），（6R，7R）-bisabolone （1.60％-4.28％），curlone（6.80％-20.63％）. PCA analysis showed that accumulative contribution rate of former 6 principle components was 100％. Cluster analysis showed that S1，S2， S6 was clustered into a category ，respecrively；and others intoa category. Total content of total tumerone decreased first and then increased as the increase of latitude ，which was the highest in Mianyang of Sichuan province （64.28％）and the lowest in Zhangzhou of Fujian province （26.92％）. CONCLUSIONS ： There are difference in composition and content of methanol and petroleum ether fractions in C. longa from different habitats.